High-cell-density cultivations to increase MVA virus production

Engineering Escherichia coli to increase plasmid DNA production in high cell-density cultivations in batch mode

BACKGROUND Plasmid DNA (pDNA) is a promising molecule for therapeutic applications. pDNA is produced by Escherichia coli in high cell-density cultivations (HCDC) using fed-batch mode. The typical limitations of such cultivations, including metabolic deviations like aerobic acetate production due to the existence of substrate gradients in large-scale bioreactors, remain as serious challenges for...

Enhanced malic acid production from glycerol with high-cell density Ustilago trichophora TZ1 cultivations

BACKGROUND In order to establish a cost-efficient biodiesel biorefinery, valorization of its main by-product, crude glycerol, is imperative. Recently, Ustilago trichophora TZ1 was found to efficiently produce malic acid from glycerol. By adaptive laboratory evolution and medium optimization, titer and rate could be improved significantly. RESULTS Here we report on the investigation of this st...

Increasing the predictivity of kinetic models for high-cell-density cultivations

In this work, an optimization-based approach is presented which recognises the switching to new parameters or even to a different model at a certain growth rate improving the quality of model prediction for different time horizon lengths. For the dynamic automatic adjustment to changing kinetics, a moving horizon estimator (MHE) is used. Experimental data from cultivation of Ustilago maydis are...

Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks

BACKGROUND Here we describe a novel cultivation method, called EnBasetrade mark, or enzyme-based-substrate-delivery, for the growth of microorganisms in millilitre and sub-millilitre scale which yields 5 to 20 times higher cell densities compared to standard methods. The novel method can be directly applied in microwell plates and shake flasks without any requirements for additional sensors or ...

Microbial Biodiesel Production - Oil Feedstocks Produced from Microbial Cell Cultivations